Figure 5

MiR-320a and miR-140 inhibit MEV infection by preventing the virus to entry into F81 cells. (A) qPCR was used to assess the effects of miR-320a and miR-140 mimics of MEV genomic DNA at the indicated times after 48 h transfection with the mimics. Data are from 3 independent experiments (mean ± SD). Statistical significance was analyzed by two-way ANOVA test; *P <0.05; **P <0.01; ***P <0.001. (B,C,D,E) Flow cytometric analysis was used to assess the effects of miR-320a and miR-140 mimics on MEV-infected F81 cells at the indicated times after 48 h transfection with the mimics. The mean fluorescence intensities (MFI) of MEV-infected F81 cells at the indicated times are shown. Data are from 3 independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test; *P <0.05; **P <0.01; ***P <0.001.