Figure 3

MEV infection leads to gradual changes of miR-320a, miR-140 and TfR in F81 cells. qPCR was used to assess the relative expression of miR-320a, miR-140 and TfR mRNAs at the indicated times (A) under normal conditions or (B) after MEV infection, with β-actin as an internal control. Data are from 3 independent experiments (mean ± SD). (C) Western blot assay was used to assess the TfR expression levels under normal conditions (−) or after MEV infection (+). (D) Western blot assay was used to assess the TfR expression levels when transfection with miRNA inhibitors post 12 h MEV infection.