Figure 2

MiR-320a and miR-140 inhibits TfR expression in F81 cells. (A) qPCR was used to assess effects of predicted miRNA mimics and inhibitors on the relative expression of TfR after 36 h transfection with either mimics or inhibitors, with β-actin as an internal control. (B) Western blot assay was used to assess the TfR protein levels of lysates of F81 cells after 48 h transfection with either mimics or inhibitors. (C) Flow cytometric analysis was used to assess the percentage of TfR-positive F81 cells after 48 h transfection with miR-320a and miR-140 mimics. (D) qPCR was used to assess the TfR mRNA levels after 36 h transfection with miR-320a and miR-140 mimics in a dose-dependent manner (E) Western blot assay was used to assess the TfR protein levels after 48 h transfection with miR-320 and miR-140 mimics in a dose-dependent manner. Data are from 3 independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test; *P <0.05; **P <0.01; ns, not significant.