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Table 2 Sequences of oligonucleotides used for construction of libraries

From: Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells

Primer

Nucleotide sequence (5 to 3′)

RT-VH

GGAGGAGACGATGACTTC

RT-VL

TTATAGGACGGTCAGGGTTGTC

VH-P1

ATGAGCCCACTCGTCTC

VH-P2

AGAGCCACCTCCGCCTGAACCGCCTCCACCGGAGGAGACGATGACTTC

VL-P1

GGCGGAGGTGGCTCTGGCGGTGGCGGGTCGGCGCTGACTCAGCCGT

VL-P2

TTATAGGACGGTCAGGGTTGTC

VHp1-NdeI

GGGAATTCCATATGGCCGTGACGTTGGAC

VLp2-BamHI

CGCGGATCCTTATAGGACGGTCAGGGTTGTC

IRES-P1

AGATCT ATGAGCCCACTCGTCTCCTCCCTCCTGCTCCTGGCCGCCCTGCCAGGGCTGATGGCG

 

GCCGTGACGTTGGACG

IRES-P2

TTACAGATCCTCTTCAGAGATGAGTTTCTGCTC GCCGGC TAGGACGGTCAGGGTTG

  1. Synthetic linker used for overlap extension PCR shown underlined.
  2. Restriction enzyme sites used for cloning shown in bold.
  3. Signal peptide sequence and c-Myc epitope tag sequence shown italics.
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Virology Journal

ISSN: 1743-422X