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Figure 4 | Virology Journal

Figure 4

From: Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells

Figure 4

siRNA in combination with scFv1 against FJ13 virus in DF-1 cells. (A) Transient transfection of NP604 and DF-1 cells by pIRES2-scFv1. The negative control was transfected by pIRES2-EGFP. (B) Expression of scFv1 in NP604 and DF-1 cells detected by western blot. (C) mRNA levels of AIV FJ13 analyzed by qPCR from 24 h to 60 h p.i. in DF-1, NP604, scFv and scFv-NP604 cells. All measurements were normalized to expression of β-actin genes, and the copies of HA gene mRNAs were quantified by comparison with a standard curve derived from known amounts of pEASY-HA. (D) All cells were infected with 100 CCID50 of FJ13. Virus titers of supernatant samples collected at 60 h p.i. were determined as CCID50 in normal MDCK cells. Data are from 3 independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test; *P <0.05; **P <0.01; ns, not significant.

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Virology Journal

ISSN: 1743-422X