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Figure 2 | Virology Journal

Figure 2

From: Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells

Figure 2

Screening of scFvs by ZDOCK module of DiscoveryStudio software. (A) Homology modeling of HA protein and scFvs. (B) Molecular docking between scFvs and HA protein. (C) The high-affinity scFvs were confirmed again by yeast two-hybrid assay. Chromogenic results showed scFv1, scFv2 and scFv3 were all positive and the blank controls were negative. (D) Expression of HA and scFvs in yeast as detected by western blot. Upper row: detection of HA expression by anti-Myc Epitope Tag.; Lower row: detection of scFvs expression by anti-HA Epitope Tag.

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Virology Journal

ISSN: 1743-422X