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Figure 1 | Virology Journal

Figure 1

From: Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells

Figure 1

Yeast two-hybrid assay and chromogenic results. (A) Constructs used in the yeast two-hybrid assays for screening of specific scFvs against HA protein. A cDNA library of scFvs was subcloned into a pGADT7 yeast two-hybrid prey vector for fusion to the GAL4 transactivation domain (AD). (B) Screening for specific scFvs against HA. Yeast clones containing bait and prey vectors were selected on SD/-Leu/-Trp/-His/-Ade plate. Fourteen positive scFv fragments appeared blue through X-gal staining.

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Virology Journal

ISSN: 1743-422X