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Table 2 Primers uesd for amplifying cytokines

From: Construction and in vitro evaluation of a recombinant live attenuated PRRSV expressing GM-CSF

Gene Upper primer (5′-3′) Lower primer (5′-3′)
GAPDH ATG GTG AAG GTC GGA GTG AAC CGT GGG TGG AAT CAT ACT GG
GM-CSF AAG CCC TGA GCC TTC TAA ACA GGT CAA ACA TTT CAC AGA CGA
IL-1β GCC CCA AAG AGA TGA AGT GCT TCC ACT GCC ACG ATG ACA GA
IL-4 GCA CAT CTA CAG ACA CCA CAC CTT TAG CCT TTC CAA GAA GTC
IL-12 GCC TGC TTA CCA CTT GAA CT GCA CAG GGT TGT CAT AAA AGA G
IFN-γ GCT CTG GGA AAC TGA ATG ACT TGA CTT CTC TTC CGC TTT CTT A
TNF-α ACC ACG CTC TTC TGC CTA CT GGC TTT GAC ATT GGC TAC AAC