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Table 1 Primers uesd for construction of recombinant PRRSV infectious clones

From: Construction and in vitro evaluation of a recombinant live attenuated PRRSV expressing GM-CSF

Primer

Nucleotide sequence (5′-3′)

Sense

Relative position in the genome

F1

TTCCGGAGACAGTCTTCAGC

+

11143bp-11162bp

R1

TTCTGCAGCCACATTTCAATTCAGGCCTAAAGTT

-

11963bp-11982bp

F2

AACTTTAGGCCTGAATTGAAATGTGGCTGCAGAA

+

11963bp-11982bp

R2

TTGCATAGACCCCATTTCATCGTTCCGCTGAAACTCTGGTTAAAGGGGTTGCCGCGGAAC TTACTTTTTGACTG

-

11983bp-12002bp

F3

GTTTCAGCGGAACGATGAAATGGGGTCTATGCAA

+

11983bp-12002bp

R3

GAAAGGCCTCATAAGATCTTCT

-

12196bp-12217bp

  1. Bold letters (nucleotides) in sequences denote the restriction enzyme sites (F1-BspE I;R3-Bgl II), and the italics in the sequence indicate the transcription-regulating sequence 6 of PRRSV(TRS6).
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Virology Journal

ISSN: 1743-422X