Figure 1

Confocal images of Vero cells infected with HeV and fixed at 8 hpi (a, b, c, e) and 18 hpi (d, f. (a, b) Cells were labelled with anti-HeV G mAb m102.4 [41] and mouse anti-PDI detected with species-specific immunoglobulins conjugated to Alexa 568 (HeV G red: a, b) and Alexa 488 (PDI; green, b, c) Cells were labelled with anti-HeV M antibody detected with anti-mouse antibody conjugated to Alexa 488 (green) showing M protein mainly in the nucleolus. d) Vero cells infected with HeV and fixed at 18 hpi. HeV M protein (green) was present in the nucleus but also throughout the cytoplasm and at the cell membrane. HeV G protein (labelled as in a, b: red) was present throughout the cell cytoplasm. e) HeV N protein (Alexa 488: green) was present at 8 hpi in punctate regions throughout the cytoplasm and in large accumulations at 18 hpi (f). Nuclei were labelled with DAPI (blue). Fluorescence images were merged with DIC images. Scale bars: a, b) =10 μm, c) =15 μm, d) =20 μm, e) =10 μm, f) =20 μm.