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Table 1 Primers used in PCR amplifications, cloning and mutagenesis of IBDV segments A and B

From: Simultaneous alteration of residues 279 and 284 of the VP2 major capsid protein of a very virulent Infectious Bursal Disease Virus (vvIBDV) strain did not lead to attenuation in chickens

Primer designation

Sequence (5'-3')

Orientation

Location

Restriction site

IB2SP1

CATGGTACC ATGACAAACCTGCAAGATCAAACC

+

131-154

Kpn I

SAR1

AGAGAATTC AGGGGACCCGCGAACGGATCCAATT

-

3237-3261

Eco RI

MutS

GCAA ACAATGGGCTAACGA CCGGCACTGACAACC

-

962-995

-

MutR

GGT CGTTAGCCCATTGTT TGCGGCCACAGCTCTG

-

949-982

-

SAR2

CAAGAATCCCGTCGAC TACG

-

1739-1720

Sal I

SAS1

GGATACGATCGGTCTGACCC

+

1-20

-

SBS1

GGATACGATGGGTCTGACCC

+

1-20

-

SBR1

GGGGCCCCCGCAGGCGAAGG

-

2826-2807

-

  1. Restriction enzyme sites are italicized.
  2. Nucleotides used for mutagenesis of VP2 residues 279 and 284 are indicated in bold.
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Virology Journal

ISSN: 1743-422X