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Figure 8 | Virology Journal

Figure 8

From: Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis

Figure 8

The VLPs prepared in pCAGGS/G-FLAG and pCAGGS/F-cmyc co-transfected cells contained actin. Cells were co-transfected with pCAGGS/G-FLAG and pCAGGS/F-cmyc, and VLPs prepared by discontinuous centrifugation as described in methods. The 0-20, 20-50 and 50-60% sucrose interface fractions were examined by immunoblotting using (A) anti-FLAG, (B) anti-cmyc and (C) anti-actin. Protein species corresponding in size to the recombinant G-FLAG and F-cmyc proteins and actin are indicated. G indicates monomeric G protein and G* is a larger G protein species.

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Virology Journal

ISSN: 1743-422X