Skip to main content
Figure 5 | Virology Journal

Figure 5

From: Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis

Figure 5

Co-localisation of recombinant expressed G and F proteins with phalloidin-FITC in LLC-MK2 cells. (A to D) LLC-MK2 cells were transfected with pCAGGS/G-FLAG and at 14 hrs post-transfection the cells were stained using anti-FLAG and phalloidin-FITC, and examined by confocal microscopy at a focal plane that allows (A and B) the cell periphery and (C and D) the cell top to be visualised. (B) is an enlarged region from plate (A) (highlighted by white box) and (D) is an enlarged region from plate (C) (highlighted by white box). (E and F) LLC-MK2 cells were co-transfected with pCAGGS/G-FLAG and pCAGGS/F-cmyc and at 14 hrs post-transfection the cells were stained using anti-cmyc and phalloidin-FITC. (F) is an enlarged region from plate (E) (highlighted by white box). In each plate the filamentous staining pattern is highlighted (white arrows).

Back to article page

Virology Journal

ISSN: 1743-422X