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Figure 4 | Virology Journal

Figure 4

From: Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis

Figure 4

Human metapneumovirus (HMPV) morphogenesis occurs within membrane structures enriched in lipid-rafts and F-actin. At 7 days post-infection HMPV-infected LLC-MK2 cells were co-stained with phalloidin-FITC and anti-G and imaged using confocal microscopy at a focal plane that allowed visualisation of the (A) cell periphery and (B) cell top. (C) is an enlarged image of the cell viewed in (B). (D) HMPV-infected LLC-MK2 cells were stained with CTX-B-AF488 and anti-G and imaged using confocal microscopy. In each case yellow staining pattern in the merged image indicates regions of co-localisation. The virus filaments (white arrows) and non-infected cells (*) are highlighted.

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