Table 1 Oligonucleotides used for specific PCR systems and resulting C q -values for rhabdoviral sequences of German bats
From: Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
Assay | Primer | Position (nt)* | Sequence (5′-3′) in the L-gene | Cq- value | Reference |
|---|---|---|---|---|---|
Rhabdo 1 | rhabdo-L1-F | 6-139 | GAGAGTATTTTGTTGTAACTGAGC | 28.5 | KJ614426 |
rhabdo-L1-R | GTCTAGACCTTGTCCATTGGA | ||||
Rhabdo2 | rhabdo-L2-F | 3-128 | TGAGGGAGTATTTCGTGATGAC | 30.3 | KJ614425 |
rhabdo-L2-R | GTCCGCTTGATGTTTCCAGC | ||||
Rhabdo3 | rhabdo-L3-F | 4-147 | GAGAGACTACTTTGTCATCACTG | 28.6 | KJ614422 |
rhabdo-L3-R | TCGTAATCATCTAGACCTTGTCC | ||||
Rhabdo4 | rhabdo-L4-F | 4-123 | GAGGGATTACTTTGTAATAACTGAG | 31.8 | KJ614427 |
rhabdo-L4-R | AGCCTTGGCCATTTGATGTATTC | ||||
Rhabdo5 | rhabdo-L5-F | 3-147 | TTCGCGACTACTTCGTCATAAC | 25.9 | KJ614424 |
rhabdo-L5-R | TCGTAATTGTTTAATCCCTGTCC | ||||
Rhabdo6 | rhabdo-L6-F | 1-134 | GCTGAGGGATTACTTTGTAATTAC | 34.6 | KJ614421 |
rhabdo-L6-R | ATCCTTGTCCATTTGACGTGTC | ||||
Rhabdo7 | rhabdo-L7-F | 1-140 | GTTACGAGATTACTTTGTGATCAC | 35.8 | KJ614423 |
rhabdo-L7-R | TGTCCAGGCCTTGTCCATTG |