Figure 5

Effects of pimprinethine, WS-30581 A and WS-30581 B on EV71 replication in RD cells. RD cells infected with 100 TCID₅₀ of EV71 were incubated in the absence (VC) or presence of 80 μM pimprinethine, 40 μM WS-30581 A or 40 μM WS-30581 B and harvested at the indicated times pi. (A) The progeny viral yields were determined. Dashed lines indicate virus titer less than the detectable dose. (B) The total RNA was extracted from cells and culture supernatants and EV71 RNA levels were measured. Cellular actin amplification was used for normalization. The △△CT data were calculated from three independent experiments. *P <0.05, compared with the virus control group. (C) EV71-protein was determined by indirect immunofluorescence using a mouse anti-enterovirus 71 monoclonal antibody and an Alexa Fluor 488-Conjugated Affinipure Goat Anti-Mouse IgG (H + L). The nucleus was stained with DAPI and the green foci indicate the presence of EV71 protein.