Figure 4

Viral lifecycle changes after CytD/CuE treatment. (A) Effect of microfilament depolymerisation and polymerization on viral invasion. To modify microfilaments, IPEC-J2 cells were treated with CytD or CuE for 2 h. Cells were then infected with PCV2 at 3×10^2.5 TCID₅₀/ml for 1 h and harvested. Viral DNAs were quantitated by qRT-PCR. (B) Effect of microfilament depolymerisation and polymerization on viral replication. IPEC-J2 cells were infected with PCV2 at 3×10^2.5 TCID₅₀/ml. To modify microfilaments, CytD or CuE was added at 1 h p.i., and supernatants and cells were harvested at 24, 48 and 72 h p.i. respectively. Viral DNAs in supernatants and cells were quantitated by qRT-PCR assay. (C) Effect of microfilament depolymerisation and polymerization on viral release. IPEC-J2 cells were infected with PCV2 at 3×10^2.5 TCID₅₀/ml. To modify microfilaments, CytD or CuE was added at 1 h p.i., and supernatants were harvested at 24, 48 and 72 h p.i. respectively. Viral DNAs in supernatants were quantitated by qRT-PCR. Asterisks above columns indicate highly significant differences between groups (P <0.01) (n = 3).