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Figure 2 | Virology Journal

Figure 2

From: Infection of Porcine Circovirus 2 (PCV2) in Intestinal Porcine Epithelial Cell Line (IPEC-J2) and Interaction between PCV2 and IPEC-J2 Microfilaments

Figure 2

Microfilament changes observed by fluorescence microscopy. IPEC-J2 cells were infected with PCV2 at 3×102.5 TCID50/ml. For actin staining at 1, 24, 48 and 72 h p.i., cells were fixed with paraformaldehyde and permeabilized with Triton X-100. F-actin was detected by phalloidin-FITC. DAPI was used as a nuclear counterstain. Images were obtained using a fluorescence microscope (ZEISS Observer.Z1). Magnification 400x.

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