Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections

Figure 2

CVB3 2A prois identified as the viral component that induces SG formation in HeLa cells. (A) HeLa cells were co-transfected with the pmCherry-HuR plasmid and one of the following plasmids: pEGFP-2A, pEGFP-2B, pEGFP-2C, pEGFP-3A, pEGFP-3B, pEGFP-3C, pEGFP-3D, pEGFP-VP1, or pEGFP-VP4-3. At 24 h post-transfection, the cells were imaged. The quantification of SG formation for each group was determined by analyzing the percentage of EGFP-expressing cells that contained three or more granules. At least 100 cells were counted for each experiment. The average of three independent experiments is indicated on the lower part of each panel. (B) HeLa cells were transfected with pEGFP-C1, pEGFP-2A, or pEGFP-3C. The cells were then fixed at 24 h post-transfection and stained for HuR via an anti-HuR rabbit polyclonal antibody and a CF555-labeled goat anti-rabbit secondary antibody. (C) HeLa cells were treated as discribed in (B) and then stained for G3BP1 via an anti-G3BP1 mouse monoclonal antibody and a CF555-labeled goat anti-mouse secondary antibody. Hoechst 33342 was used to counterstain the nuclei. The SGs were determined by observing the granular distribution of mCherry-HuR (A), HuR (B), and G3BP1 (C) in the cytoplasm under a fluorescence microscope (×400). The cytoplasmic granules are indicated by arrow heads.

Back to article page