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Figure 1 | Virology Journal

Figure 1

From: Osteoblasts from osteoarthritis patients show enhanced susceptibility to Ross River virus infection associated with delayed type I interferon responses

Figure 1

Enhanced RRV replication in primary hOBs of OA patients. (A) Phenotypic characterization of primary hOBs. Primary hOBs were fixed and stained for osteocalcin (green) and nuclei (blue), and visualized by confocal microscopy. Magnification, ×20. Images are representative of 2 independent experiments. Primary hOBs were infected with RRV-EGFP at MOI 1 and cells and supernatant were harvested at different time points. (B) Dose-dependent infection of hOBs. Cell harvested at 24 hpi were fixed, stained for 7-AAD, and subjected to FACS analysis. FACS scatter plots are representative of the data obtained for 3 independent experiments. (C) Quantification of percentage EGFP+ cells after infection. (D) Supernatant was harvested and RRV titre was determined by plaque assay on Vero cells. Data (n =4) are presented as mean ± SEM. *P <0.05, two-way ANOVA, Bonferroni post-test.

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Virology Journal

ISSN: 1743-422X