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Figure 1 | Virology Journal

Figure 1

From: Anti-herpes virus activities of bioactive fraction and isolated pure constituent of Mallotus peltatus: an ethnomedicine from Andaman Islands

Figure 1

IR,1HNMR and Mass Spectra of isolated compound from fraction A with authentic ursolic acid. The concentrated crude methanolic extract was partitioned between n-butanol and water. The aqueous part was lyophilized while the solvent part was evaporated by Eyela (Uni trap UT 1000, Japan) rotary evaporator at 45°C. The n-butanol fraction was purified on silica gel column, and eluted with PE: PE: CHCl3, CHCl3: MeOH and MeOH. The eluted fractions, monitored by TLC, yielded two major condensed fractions A and B. The isolated compound(s) from fraction A was then purified by repeated silica gel column and eluted with PE: CHCl3 (1:1) and CHCl3: MeOH (95:5) mixture to obtain pure compound. The IR spectra of isolated compounds from fraction A done by JASCO-FTIR spectrophotometer in potassium bromide discs [A] agreed well with the authentic sample of ursolic acid [B]. The 1HNMR spectrum of isolated compound from fraction A by Bruker DPX-300 NMR spectrometer in DMSO-d6 solution [C] indicated that the isolated compound was almost identical with the authentic sample of ursolic acid [D]. The Mass spectra of isolated compound from fraction A, determined by JEOL JMS600 Mass Spectrometer [E] indicated the compound to be ursolic acid.

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