Viruses
|
IVPI
|
Virus replication in chickens#(log10EID50/ml ± SE)
|
MLD50
(log10EID50
/50 μl)
|
Virus replication in mice&(log10EID50/ml ± SE)
|
---|
Day 3
|
Day 5
| | |
---|
Trachea
|
Cloacal
|
Lungs
|
Trachea
|
Cloacal
|
Lungs
|
Day 3
|
Day 6
|
---|
C4/W1
|
0.8
|
4/4* (3.9 ± 0.4)
|
4/4 (2.4 ± 0.5)
|
4/4 (3.5 ± 0.2)
|
4/4 (5.4 ± 0.2)
|
3/4 (3.3 ± 0.3)
|
4/4 (4.7 ± 0.6)
|
6.5
|
3/3* (4.6 ± 0.2)
|
3/3 (3.3 ± 0.3)
|
ts-C4/W1
|
0
|
0/4
|
0/4
|
0/4
|
0/4
|
0/4
|
0/4
|
>8.0
|
2/3 (1.6 ± 0.1)
|
0/3
|
C4/W1-PA(F35S)
|
0
|
4/4 (1.8 ± 0.3)
|
0/4
|
0/4
|
0/4
|
0/4
|
0/4
|
>8.0
|
3/3 (3.4 ± 0.3)
|
2/3 (2.5 ± 0.1)
|
- Note: IVPI, intravenous pathogenicity index; MLD50, 50% mouse lethal dose; EID50, 50% egg infectious dose; SE, standard errors.
- # Groups of chickens were inoculated intranasally with 0.2 ml 106 EID50 of the indicated viruses. At 3 and 5 days post-inoculation, viral titers in tracheal swabs, cloacal swabs and lungs were determined by testing their EID50 at 37°C, values are the means ± standard errors.
- &Groups of mice were inoculated intranasally with 50 μl 106 EID50 of the indicated viruses. At 3 and 6 days post-inoculation, viral titers in lungs were determined by testing their EID50 at 37°C, values are the means ± standard errors.
- * NO. of chickens or mice with detectable virus/total chickens or mice used.