Skip to main content

Advertisement

Table 1 Pathogenicity and growth property of recombinant viruses in chickens and mice

From: The new temperature-sensitive mutation PA-F35S for developing recombinant avian live attenuated H5N1 influenza vaccine

Viruses IVPI Virus replication in chickens#(log10EID50/ml ± SE) MLD50 (log10EID50 /50 μl) Virus replication in mice&(log10EID50/ml ± SE)
Day 3 Day 5   
Trachea Cloacal Lungs Trachea Cloacal Lungs Day 3 Day 6
C4/W1 0.8 4/4* (3.9 ± 0.4) 4/4 (2.4 ± 0.5) 4/4 (3.5 ± 0.2) 4/4 (5.4 ± 0.2) 3/4 (3.3 ± 0.3) 4/4 (4.7 ± 0.6) 6.5 3/3* (4.6 ± 0.2) 3/3 (3.3 ± 0.3)
ts-C4/W1 0 0/4 0/4 0/4 0/4 0/4 0/4 >8.0 2/3 (1.6 ± 0.1) 0/3
C4/W1-PA(F35S) 0 4/4 (1.8 ± 0.3) 0/4 0/4 0/4 0/4 0/4 >8.0 3/3 (3.4 ± 0.3) 2/3 (2.5 ± 0.1)
  1. Note: IVPI, intravenous pathogenicity index; MLD50, 50% mouse lethal dose; EID50, 50% egg infectious dose; SE, standard errors.
  2. # Groups of chickens were inoculated intranasally with 0.2 ml 106 EID50 of the indicated viruses. At 3 and 5 days post-inoculation, viral titers in tracheal swabs, cloacal swabs and lungs were determined by testing their EID50 at 37°C, values are the means ± standard errors.
  3. &Groups of mice were inoculated intranasally with 50 μl 106 EID50 of the indicated viruses. At 3 and 6 days post-inoculation, viral titers in lungs were determined by testing their EID50 at 37°C, values are the means ± standard errors.
  4. * NO. of chickens or mice with detectable virus/total chickens or mice used.