GRA treatment reduces the levels of VP2, VP6 and NSP2 in infected cells. Cells were inoculated with RRV at a multiplicity of infection of 3 pfu/cell. Virus was adsorbed for one hour at 4°C, and cultures then were shifted to 37°C for one hour (A) or two hours (B) prior to addition of GRA. Lysates were probed in immunoblots with anti-rotavirus strain SA11F antiserum to detect VP2 and VP6, anti‒NSP2, or anti‒GAPDH as a loading control. The lanes are triplicate samples. Bands were quantified by densitometry taking the averaged intensity of the bands in the individual samples normalized to the averaged intensity of the GAPDH loading control in each sample.