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Table 1 Primers used for real-time PCR, real-time RT-PCR, and LMD and RT-PCR

From: Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek’s disease virus

Genes

Analysis

Types

Sequences

Annealing temperature for LMD and RT-PCR analysis

Accession number

meq

real-time PCR

Forward

5′-GTCCCCCCTCGATCTTTCTC-3′

60°C

AY362736

 

real-time RT-PCR

Reverse

5′-CGTCTGCTTCCTGCGTCTTC-3′

  
 

LMD and RT-PCR

    

β-actin

real-time PCR

Forward

5′-GAGAAATTGTGCGTGACATCA-3′

55°C

L08165

  

Reverse

5′-CCTGAACCTCTCATTGCCA-3′

  

β-actin

real-time RT-PCR

Forward

5′-CCAACTGGGATGATATGGAGAAG-3′

58°C

NM205518

 

LMD and RT-PCR

Reverse

5′-AGGCATACAGGGACAGCACA-3′

  

PD-1

real-time RT-PCR

Forward

5′-GGACTACGGTGTGCTGGAGTT-3′

60°C

XM422723

 

LMD and nested RT-PCR (2nd PCR)

Reverse

5′-TCTTTCCTCGCTCTGGTGTG-3′

  

PD-1

LMD and nested RT-PCR (1st PCR)

Forward

5′-ACACCCTGGCGGAGGTGAAG-3′

60°C

XM422723

(1st PCR)

 

Reverse

5′-TGCCAGGTCCTCTGATCGTTGTG-3′

  

PD-L1

real-time RT-PCR

Forward

5′-TTCAGGGACGGATAAAGCTG-3′

60°C

XM424811

 

LMD and nested RT-PCR (2nd PCR)

Reverse

5′-CGTCTCTGAGCTTCACGTTG-3′

  

PD-L1

LMD and nested RT-PCR (1st PCR)

Forward

5′-GGGACTTAAGCGTCATCTGGGAA-3′

58°C

XM424811

(1st PCR)

 

Reverse

5′-GGTAAAGCCCCGCGTCTCTG-3′

  

IFN-γ

real-time RT-PCR

Forward

5′-CTCCCGATGAACGACTTGAG-3′

58°C

NM205149

  

Reverse

5′-CTGAGACTGGCTCCTTTTCC-3′