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Table 1 Primers used for real-time PCR, real-time RT-PCR, and LMD and RT-PCR

From: Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek’s disease virus

Genes Analysis Types Sequences Annealing temperature for LMD and RT-PCR analysis Accession number
meq real-time PCR Forward 5′-GTCCCCCCTCGATCTTTCTC-3′ 60°C AY362736
  real-time RT-PCR Reverse 5′-CGTCTGCTTCCTGCGTCTTC-3′   
  LMD and RT-PCR     
β-actin real-time PCR Forward 5′-GAGAAATTGTGCGTGACATCA-3′ 55°C L08165
   Reverse 5′-CCTGAACCTCTCATTGCCA-3′   
β-actin real-time RT-PCR Forward 5′-CCAACTGGGATGATATGGAGAAG-3′ 58°C NM205518
  LMD and RT-PCR Reverse 5′-AGGCATACAGGGACAGCACA-3′   
PD-1 real-time RT-PCR Forward 5′-GGACTACGGTGTGCTGGAGTT-3′ 60°C XM422723
  LMD and nested RT-PCR (2nd PCR) Reverse 5′-TCTTTCCTCGCTCTGGTGTG-3′   
PD-1 LMD and nested RT-PCR (1st PCR) Forward 5′-ACACCCTGGCGGAGGTGAAG-3′ 60°C XM422723
(1st PCR)   Reverse 5′-TGCCAGGTCCTCTGATCGTTGTG-3′   
PD-L1 real-time RT-PCR Forward 5′-TTCAGGGACGGATAAAGCTG-3′ 60°C XM424811
  LMD and nested RT-PCR (2nd PCR) Reverse 5′-CGTCTCTGAGCTTCACGTTG-3′   
PD-L1 LMD and nested RT-PCR (1st PCR) Forward 5′-GGGACTTAAGCGTCATCTGGGAA-3′ 58°C XM424811
(1st PCR)   Reverse 5′-GGTAAAGCCCCGCGTCTCTG-3′   
IFN-γ real-time RT-PCR Forward 5′-CTCCCGATGAACGACTTGAG-3′ 58°C NM205149
   Reverse 5′-CTGAGACTGGCTCCTTTTCC-3′