|
Antigenic Sitea
|
pb from aligned sequencesc (pb from resorted sequencesd)
|
|---|
|
Δabs
|
ΔΔASAtot
|
ΔΔASAnp
|
ΔΔASApol
|
ΔQ
|
|---|
|
H1 epitope residues vs. randome
|
|---|
|
Ca2
|
p < 0.05 (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (p < 0.05)
|
|
Sb
|
p < 0.05 (p < 0.01)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (NS)
|
|
Sa
|
NS (p < 0.05)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
H1C
|
p < 0.01 (p < 0.05)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
Ca1
|
NS (NS)
|
NS (p < 0.01)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
Cb
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
H3 epitope residues vs. randomf
|
|
A
|
NS (p < 0.05)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
B
|
p < 0.05 (p < 0.01)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
C
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
D
|
p < 0.05 (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
|
E
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (NS)
|
|
B HA epitope residues vs. randomg
|
|
BA
|
NS (p < 0.01)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (NS)
|
|
BB1
|
NS (p < 0.01)
|
NS (NS)
|
NS (p < 0.05)
|
NS (NS)
|
p < 0.001 (p < 0.05)
|
|
BB2
|
NS (p < 0.05)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (NS)
|
|
BC
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (NS)
|
|
BD
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
p < 0.05 (NS)
|
|
BE
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
NS (NS)
|
- aSee Table 2
- bProbability determined from comparison of antigenic site residues to randomly selected residues (see below) determined using Kruskal-Wallis ANOVA
- cAmino acid sequences aligned using MUSCLE. See Additional file 1 for resultant sequence alignment.
- dAntigenic site residues, along with a set of randomly selected residues (below), were extracted from each sequence, then the datasets containing the extracted residues representing each sequence were re-organized and Δabs, ΔΔASAtot, ΔΔASAnp, and ΔQ recalculated for each amino acid in the dataset based on the new arrangement of sequences (see Methods)
- eRandomly selected H1 residues: 2, 19, 23, 76, 84, 94, 117, 121, 178, 223, 301, 326
- fRandomly selected H3 residues: 12, 72, 111, 123, 139, 179, 222, 289, 312, 328
- gRandomly selected influenza B HA residues: 4, 17, 36, 47, 78, 152, 196, 222, 251, 273, 300, 304
