Subtype- and antigenic site-specific differences in biophysical influences on evolution of influenza virus hemagglutinin

Table 4 Effect of partial randomization of sequence dataset on antigenic site statistics

Antigenic Site^a	p^b from aligned sequences^c (p^b from resorted sequences^d)
Antigenic Site^a	Δabs	ΔΔASAtot	ΔΔASAnp	ΔΔASApol	ΔQ
H1 epitope residues vs. random^e
Ca2	p < 0.05 (NS)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (p < 0.05)
Sb	p < 0.05 (p < 0.01)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (NS)
Sa	NS (p < 0.05)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
H1C	p < 0.01 (p < 0.05)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
Ca1	NS (NS)	NS (p < 0.01)	NS (NS)	NS (NS)	NS (NS)
Cb	NS (NS)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
H3 epitope residues vs. random^f
A	NS (p < 0.05)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
B	p < 0.05 (p < 0.01)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
C	NS (NS)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
D	p < 0.05 (NS)	NS (NS)	NS (NS)	NS (NS)	NS (NS)
E	NS (NS)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (NS)
B HA epitope residues vs. random^g
BA	NS (p < 0.01)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (NS)
BB1	NS (p < 0.01)	NS (NS)	NS (p < 0.05)	NS (NS)	p < 0.001 (p < 0.05)
BB2	NS (p < 0.05)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (NS)
BC	NS (NS)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (NS)
BD	NS (NS)	NS (NS)	NS (NS)	NS (NS)	p < 0.05 (NS)
BE	NS (NS)	NS (NS)	NS (NS)	NS (NS)	NS (NS)

^aSee Table 2
^bProbability determined from comparison of antigenic site residues to randomly selected residues (see below) determined using Kruskal-Wallis ANOVA
^cAmino acid sequences aligned using MUSCLE. See Additional file 1 for resultant sequence alignment.
^dAntigenic site residues, along with a set of randomly selected residues (below), were extracted from each sequence, then the datasets containing the extracted residues representing each sequence were re-organized and Δabs, ΔΔASAtot, ΔΔASAnp, and ΔQ recalculated for each amino acid in the dataset based on the new arrangement of sequences (see Methods)
^eRandomly selected H1 residues: 2, 19, 23, 76, 84, 94, 117, 121, 178, 223, 301, 326
^fRandomly selected H3 residues: 12, 72, 111, 123, 139, 179, 222, 289, 312, 328
^gRandomly selected influenza B HA residues: 4, 17, 36, 47, 78, 152, 196, 222, 251, 273, 300, 304

ISSN: 1743-422X