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Table 1 Oligonucleotides used for the generation of bait sequences a

From: The screening and functional study of proteins binding with the BmNPV polyhedrin promoter

Cis-element repeat Primer sequence
Tandem repeat segments (3rep) 5′-CCGAAGCTTGCAAATAAATAAGTATT GCAAATAAATAAGTATT
GCAAATAAATAAGTATTCTCGAGGCCATGCCAT-3′
3rep antisense 5′ -ATGGCATGGCCTCGAGAATAC-3′
Tandem repeat mutant segments (3mut) 5′-CCGAAGCTTATGGGTGGGTGGATGTT TACTTTCAGACTTGAGTT
ACTTTCAGACTTGAGTTCTCGAGGCCATGCCAT-3′
3mut antisense 5′ -ATGGCATGGCCTCGAGC-3′
  1. aUnderlined sequences represent several repeats of the polyhedrin promoter conserved sequence ‘rep’ cis-elements and the mutant ‘3mut’. Letters in boxes represent the restriction enzymes Hin d III and Xho I.