Table 1 Oligonucleotides used for the generation of bait sequences a
From: The screening and functional study of proteins binding with the BmNPV polyhedrin promoter
Cis-element repeat | Primer sequence |
|---|---|
Tandem repeat segments (3rep) | 5′-CCGAAGCTTGCAAATAAATAAGTATT GCAAATAAATAAGTATT GCAAATAAATAAGTATTCTCGAGGCCATGCCAT-3′ |
3rep antisense | 5′ -ATGGCATGGCCTCGAGAATAC-3′ |
Tandem repeat mutant segments (3mut) | 5′-CCGAAGCTTATGGGTGGGTGGATGTT TACTTTCAGACTTGAGTT ACTTTCAGACTTGAGTTCTCGAGGCCATGCCAT-3′ |
3mut antisense | 5′ -ATGGCATGGCCTCGAGC-3′ |