Figure 2
From: Characterization of grass carp reovirus minor core protein VP4
Indirect IF and IB assays of the VP4 protein. A: Indirect IF assay of the VP4 protein in GCRV infected CIK cells. Cells were probed with the VP4 antiserum and labeled with FITC-conjugated goat anti-mouse immunoglobulin G(IgG), counterstained with hoechst to visualize the nuclei, Mock infection (upper panel); GCRV infected CIK cell (lower panel). B: IB assay of the VP4 protein in GCRV and its infected CIK cells using mouse anti-VP4 polyclonal serum as the primary antibody followed by goat anti-mouse IgG coupled to alkaline-phosphatase. M, Standard protein marker; Lane1, Recombinant His-tag fusion VP4 protein; 2–3, purified GCRV core and top component particles; 4–5, GCRV-infected and mock-infected CIK cell lysate.