Figure 2

Immunofluorescence labeling of RORγ and FoxP3 at the maternal fetal interface of tissue from representative infected cat. Negative controls included no primary antibody (top panels). RORγ and FoxP3 (green) were detected using polyclonal rabbit anti-FoxP3 or anti-RORγ antiserum followed by goat anti-rabbit IgG (H + L) fluorescein conjugate (middle panels). Cells were counterstained with DAPI (blue) (bottom panels). Cells were viewed by confocal laser scanning microscopy using a 40x oil immersion objective.