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Figure 2 | Virology Journal

Figure 2

From: Protection conferred by a recombinant Marek’s disease virus that expresses the spike protein from infectious bronchitis virus in specific pathogen-free chicken

Figure 2

Verification of the structure of the rMDVs by a series of PCR reactions. The S1 gene could be detected from both rMDV-EGFP-S1 (lane 2) and rMDV-S1 (lane 3) with primers S1-F and S1-R. The transfer vector pUP-LTR-EGFP-S1-DOWN was used as positive control (lane 1), and CVI988/Rispens DNA was used as a negative control (lane 4). The correct orientation of the inserted genes was further confirmed with the primers PLTR-F and S1-R (lanes 6 and 7). The transfer vector pUP-LTR-EGFP-S1-DOWN was used as a positive control (lane 5), and CVI988/Rispens DNA was used as a negative control (lane 8). The incorporation of the foreign gene in the correct site of rMDV-S1 genome was also verified with the following three primer pairs: Geno-F and Geno-R (lane 9), Geno-F and PLTR-R (lane 11), and S1-F2 and Geno-R (lane 12). CVI988/Rispens DNA was used as a negative control (lane 10).

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Virology Journal

ISSN: 1743-422X