Figure 4

Amino acid deletion was not necessary for Nsp2 induced activation of NF-κB.

(A) HeLa cells were co-transfected with the NF-κB-Luc reporter plasmid (0.2 μg), 0.05 μg of pRL-TK, and the designated Nsp2 expression plasmids (0.6 μg). An empty vector was used as a control. Cell extracts were collected at 36 h post-transfection and analysed for luciferase expression. (a) P < 0.05 compared with the vector group, (b) P < 0.05 compared with Nsp2 (CH-1a) protein transfection group. (B) Distinct insertion mutants or wild-type Nsp2 were transfected into HeLa cells together with NF-κB-Luc (0.2 μg) and pRL-TK (0.05 μg). Cell lysates were prepared at 36 h post-transfection and subjected to a dual-luciferase assay. There was no significant statistical difference between the wild-type Nsp2 and its mutants to activate NF-κB (P > 0.05).