Table 3 Percent inhibition of WNV-reactive MAb binding with competitive ELISA using sera from WNV-infected chicken and WNV-vaccinated horse during period between infection and serum collection
From: West Nile virus: characterization and diagnostic applications of monoclonal antibodies
| Â | Days post-infection/vaccination | WNV VN titer | % Inhibition of MAb binding | |||||
|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | 3B2 | 3D6 | 4D3 | 1C3 | 2A8 | 4G9 |
Chicken | 0 | < 5 | 0 | 4.7 ± 2.1 | 4.7 ± 1.5 | 8 ± 2 | 7 ± 1.2 | 4.3 ± 1.5 |
|  | 7 | 10 | 56.7 ± 2.1 | 76.7 ± 2.1 | 43.3 ± 2.5 | 79 ± 4.6 | 75.7 ± 1.5 | 75 ± 3.5 |
|  | 15 | 20 | 77 ± 1 | 80.3 ± 2.1 | 78.7 ± 2.5 | 86.7 ± 1.2 | 87 ± 2.6 | 91.7 ± 1.5 |
|  | 24 | 40 | 81 ± 2.6 | 80 ± 1.7 | 83.7 ± 1.5 | 89.3 ± 1.2 | 89 ± 1 | 94.3 ± 0.6 |
|  | 42 | 160 | 92.7 ± 1.5 | 95.3 ± 0.6 | 91 ± 1 | 93.7 ± 1.2 | 95.7 ± 1.2 | 94.3 ± 1.5 |
Horse | 0 | < 5 | 28.7 ± 2.1 | 15.7 ± 2.1 | 47.3 ± 2.5 | 19 ± 1 | 23.7 ± 1.5 | 23.3 ± 2.5 |
|  | 15 | < 5 | 71 ± 2 | 42.3 ± 1.2 | 73 ± 1 | 42.7 ± 0.6 | 44 ± 1 | 64.3 ± 1.5 |
|  | 21 | 10 | 81.7 ± 1.5 | 65 ± 1 | 74.7 ± 0.6 | 69.3 ± 3.5 | 65.7 ± 1.5 | 80.3 ± 2.1 |
|  | 28 | 20 | 88.7 ± 0.6 | 71.7 ± 1.5 | 85.7 ± 1.5 | 78.7 ± 1.2 | 77 ± 2 | 88.3 ± 0.6 |
|  | 31 (2°v) | 80 | 95.7 ± 0.6 | 92.7 ± 1.5 | 91.3 ± 1.2 | 91.7 ± 1.5 | 93.3 ± 3.1 | 95 ± 1 |