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Table 3 Percent inhibition of WNV-reactive MAb binding with competitive ELISA using sera from WNV-infected chicken and WNV-vaccinated horse during period between infection and serum collection

From: West Nile virus: characterization and diagnostic applications of monoclonal antibodies

  Days post-infection/vaccination WNV VN titer % Inhibition of MAb binding
    3B2 3D6 4D3 1C3 2A8 4G9
Chicken 0 < 5 0 4.7 ± 2.1 4.7 ± 1.5 8 ± 2 7 ± 1.2 4.3 ± 1.5
  7 10 56.7 ± 2.1 76.7 ± 2.1 43.3 ± 2.5 79 ± 4.6 75.7 ± 1.5 75 ± 3.5
  15 20 77 ± 1 80.3 ± 2.1 78.7 ± 2.5 86.7 ± 1.2 87 ± 2.6 91.7 ± 1.5
  24 40 81 ± 2.6 80 ± 1.7 83.7 ± 1.5 89.3 ± 1.2 89 ± 1 94.3 ± 0.6
  42 160 92.7 ± 1.5 95.3 ± 0.6 91 ± 1 93.7 ± 1.2 95.7 ± 1.2 94.3 ± 1.5
Horse 0 < 5 28.7 ± 2.1 15.7 ± 2.1 47.3 ± 2.5 19 ± 1 23.7 ± 1.5 23.3 ± 2.5
  15 < 5 71 ± 2 42.3 ± 1.2 73 ± 1 42.7 ± 0.6 44 ± 1 64.3 ± 1.5
  21 10 81.7 ± 1.5 65 ± 1 74.7 ± 0.6 69.3 ± 3.5 65.7 ± 1.5 80.3 ± 2.1
  28 20 88.7 ± 0.6 71.7 ± 1.5 85.7 ± 1.5 78.7 ± 1.2 77 ± 2 88.3 ± 0.6
  31 (2°v) 80 95.7 ± 0.6 92.7 ± 1.5 91.3 ± 1.2 91.7 ± 1.5 93.3 ± 3.1 95 ± 1
  1. MAbs were tested in triplicate. All sera were diluted 5 fold.
  2. Values are mean ± standard deviations.