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Table 2 Results of competition binding ELISAs for MAb epitope studies

From: West Nile virus: characterization and diagnostic applications of monoclonal antibodies

  Unlabelled MAbs HRP-conjugated MAbs
   3B2 HRP 3D6 HRP 1C3 HRP 4D3 HRP 2A8 HRP 4G9 HRP
Group 1 3B2 4 6 85 64 90 80
  3D6 4 5 86 65 87 75
Group 2 1C3 87 95 7 60 75 60
Group 3 4D3 85 93 91 5 88 72
Group 4 2A8 95 100 84 65 4 4
  4G9 92 100 84 63 4 3
no MAb 100 100 100 100 100 100
  1. Result expressed as percent binding of HRP-conjugated MAbs. The amount of binding obtained in the absence of unlabeled antibody was set at 100% for each HRP conjugated MAb. First column indicates the group assignments of MAbs based on competition binding assay. MAbs binding to overlapping epitopes are grouped together, while non-competing MAbs are grouped individually.