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Table 2 Results of competition binding ELISAs for MAb epitope studies

From: West Nile virus: characterization and diagnostic applications of monoclonal antibodies

 

Unlabelled MAbs

HRP-conjugated MAbs

  

3B2 HRP

3D6 HRP

1C3 HRP

4D3 HRP

2A8 HRP

4G9 HRP

Group 1

3B2

4

6

85

64

90

80

 

3D6

4

5

86

65

87

75

Group 2

1C3

87

95

7

60

75

60

Group 3

4D3

85

93

91

5

88

72

Group 4

2A8

95

100

84

65

4

4

 

4G9

92

100

84

63

4

3

no MAb

100

100

100

100

100

100

  1. Result expressed as percent binding of HRP-conjugated MAbs. The amount of binding obtained in the absence of unlabeled antibody was set at 100% for each HRP conjugated MAb. First column indicates the group assignments of MAbs based on competition binding assay. MAbs binding to overlapping epitopes are grouped together, while non-competing MAbs are grouped individually.