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Table 1 Properties of selected anti-WNV MAbs

From: West Nile virus: characterization and diagnostic applications of monoclonal antibodies

MAbs IPMA VNT WB Isotype
  WNVs USUVs TBEV WNV Eg101 WNV B956   
3B2 + - - 12800 12800 52 Kd IgG2a
3D6 + - - 12800 12800 52 Kd IgG2a
4D3 + - - 1600 1600 - IgG2b
1C3 + - - < 20 < 20 - IgG2a
2A8 + + - < 20 < 20 - IgG2a
4G9 + + - < 20 < 20 - IgG2a
  1. IPMA: Detection of WNVs, USUVs and TBEV infected Vero cells by immunoperoxidase assay. WNVs used are: Eg101, B956 ATCC, NY99 ATCC, Central African Republic 1967, 203204/08, 225677/09, 208659/09, 204913/09. USUVs used are: SAAR 1776, Vienna 2001-blackbird and 200092/2010. TBEV used is 103457/09. + indicate reactivity against all viral strains tested, - indicate absence of reactivity with all viral strains.
  2. VNT: Virus neutralization against WNV Eg101 and WNV B956. Neutralizing titers are calculated as reciprocal of MAb dilution producing 100% reduction of CPE. MAbs were used as ascites fluid.