IPMA: Detection of WNVs, USUVs and TBEV infected Vero cells by immunoperoxidase assay. WNVs used are: Eg101, B956 ATCC, NY99 ATCC, Central African Republic 1967, 203204/08, 225677/09, 208659/09, 204913/09. USUVs used are: SAAR 1776, Vienna 2001-blackbird and 200092/2010. TBEV used is 103457/09. + indicate reactivity against all viral strains tested, - indicate absence of reactivity with all viral strains.
VNT: Virus neutralization against WNV Eg101 and WNV B956. Neutralizing titers are calculated as reciprocal of MAb dilution producing 100% reduction of CPE. MAbs were used as ascites fluid.