Orthogonal inactivation of influenza and the creation of detergent resistant viral aggregates: towards a novel vaccine strategy

Table 2 Influenza is completely inactivated by INA treatment and is inactivated to varying degrees by Triton X-100

Treatment^a (Triton %, Temperature, etc)	Logs of inactivation^b
0%, R.T.	0
	0
	0
	0
0%, 37°C	0.36
	0
	0.33
	0.60
0.2%, R.T.	3.43
	3.04
0.2%, 37°C	5.56
	5.32
	5.48
	4.91
0.3%, R.T.	3.67
	3.30
0.3%, 37°C	5.48
	5.30
0.4%, R.T.	3.87
	4.13
	4.26
0.4%, 37°C	5.48
	5.30
0.5%, R.T.	3.88
	4.00
0.5%, 37°C	5.48
	5.30
0%, RT, +INA (100 uM, UVA = 5 min.)	5.56
	4.56

Duration of Triton treatment was 1 hour with periodic vortex mixing, before dilution and addition to MDCK cells for a plaque assay. Additional information is available in supplementary information
^aR.T. = room temperature. Only last two entries were INA-treated, as denoted.
^b Difference in the number of logs calculated from the room temperature control for the same lot number, taking into consideration any additional dilution effects. The limit of detection of the assay is between 5 and 6 logs of inactivation depending on the viral lot used

ISSN: 1743-422X