Skip to main content

Table 3 Primers used in this studya

From: Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host

Primers

Sequences

EXH1

AACTTTAAGAAGGAGATATACATatggttatgatgtta

EXH2

ACGCTCTAGA AATAATTTTGTTTAACTTTAAGAAGGAG

EXH3

CGCGGATCC ctttgattagtttcatttat

EXH4

CGCGGATCC cttatcttaaactggttttt

EXH5

TTAACTTTAAGAAGGAGGAAATCatgacaatcaacatt

SYB34

AACTTTAAGAAGGAGATATACATatggttatgtgatta

SYB35

ttccgatgtcagtgctt

SYB36

ttggctttgccgtagtt

GAPDH1

actatcggcttggtaatcccagaat

GAPDH2

atgaaccgaatgagatacctacga

  1. a. New restriction sites and RBS are indicated by underline and shadow, respectively. Lowercase letters represent sequences corresponding to that of HolSMP.