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Table 3 Primers used in this studya

From: Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host

Primers Sequences
EXH1 AACTTTAAGAAGGAGATATACATatggttatgatgtta
EXH2 ACGCTCTAGA AATAATTTTGTTTAACTTTAAGAAGGAG
EXH3 CGCGGATCC ctttgattagtttcatttat
EXH4 CGCGGATCC cttatcttaaactggttttt
EXH5 TTAACTTTAAGAAGGAGGAAATCatgacaatcaacatt
SYB34 AACTTTAAGAAGGAGATATACATatggttatgtgatta
SYB35 ttccgatgtcagtgctt
SYB36 ttggctttgccgtagtt
GAPDH1 actatcggcttggtaatcccagaat
GAPDH2 atgaaccgaatgagatacctacga
  1. a. New restriction sites and RBS are indicated by underline and shadow, respectively. Lowercase letters represent sequences corresponding to that of HolSMP.