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Figure 4 | Virology Journal

Figure 4

From: Highly pathogenic avian influenza virus H5N1 controls type I IFN induction in chicken macrophage HD-11 cells: a polygenic trait that involves NS1 and the polymerase complex

Figure 4

The NS1 protein of Yama controls the induction of type I IFN in HD-11 cells. HD-11 cells were infected with Yama or Yama-NS1A144V at an MOI of 0.05 TCID50/cell (A). After 24 h, supernatants were tested for type I IFN bioactivity. The standard deviations of the type I IFN values were calculated from triplicate measurements of two cultures (A). The supernatants of HD-11 cells infected with increasing MOI of Yama or Yama-NS1A144V were assayed for Mx-promoter induction in CEC-32 bioassay indicator cells (B). HD-11 (C) or DF-1 cells (D) were infected with the indicated viruses at an MOI of 0.05 TCID50/cell. At the indicated times after infection, virus titres were determined. The data are representative of two independent experiments.

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Virology Journal

ISSN: 1743-422X