Figure 3
From: Inhibition of HIV-1 replication with stable RNAi-mediated knockdown of autophagy factors
Analysis of HIV-1 replication, ATG-mRNA levels and cell growth in stable knockdown cells. A. Inhibition of HIV-1 replication as observed in Figure 2 was confirmed in newly generated knockdown cells for Beclin1 and WIPI1. CA-p24 concentration was followed for 10-11 days post infection. B. In a second experiment HIV-1 inhibition was confirmed for PIK3R4-1, Atg4A-1 and 3, Atg5-4, Atg10- 3 and 5 and Atg16-4. C. The mRNA knockdown was measured with RT-qPCR analysis for the indicated shRNAs. The level measured in the control SHC1 cells was set at 100%. D. Transduced cell mixtures were analyzed in the competitive cell growth assay, which is based on different growth rates of untransduced GFP- versus transduced GFP+ cells. Cell proliferation rates were compared to SHC1 cells, which was set at 100%.