Figure 2
From: Inhibition of HIV-1 replication with stable RNAi-mediated knockdown of autophagy factors
Screen of shRNAs against 12 autophagy factors. Stable knockdown cell lines with shRNAs against 12 ATGs were generated and challenged with HIV-1. Control cells are untransduced SupT1 cells, SHC1 cells (empty vector) and SHC2 cells, expressing a scrambled shRNA. The screen was repeated twice and each infection was performed in duplicate. The concentration of viral CA-p24 protein (ng/ml) in the culture supernatant at peak infection (day 10) was measured. CA-p24 concentrations were normalized with the Factor Correction program [39] and the average is plotted, error bars represent standard deviation from the mean.