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Figure 6 | Virology Journal

Figure 6

From: Mouse-adapted scrapie strains 139A and ME7 overcome species barrier to induce experimental scrapie in hamsters and changed their pathogenic features

Figure 6

Conformational stability of PrPSc in hamsters' brains of agent 263 K, 139A-ha and ME7-ha. (A) Western blots of total PrP with mAb 3 F4. Hamsters' brain homogenates are incubated with 1 to 6 M GdnHCl. The concentrations of GdnHCl in individual preparation are indicated on the top. Molecular weight standards are shown on the left. (B) Quantitative analyses of each gray numerical value of total PrP blots. The average values are calculated from three independent blots and presented with as mean ± SD. (C) Western blots of PrPSc with mAb 3F4. Hamsters' brain homogenates are incubated with 1 to 6 M GdnHCl and subsequently exposed to 50 μg/ml PK. The concentrations of GdnHCl in individual preparation are indicated on the top. Molecular weight standards are shown on the left. (D) Quantitative analyses of each gray numerical value of PrPSc blots. The average values are calculated from three independent blots and presented with as mean ± SD.

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Virology Journal

ISSN: 1743-422X