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Figure 4 | Virology Journal

Figure 4

From: Mouse-adapted scrapie strains 139A and ME7 overcome species barrier to induce experimental scrapie in hamsters and changed their pathogenic features

Figure 4

Analyses for PrPSc in brain homogenates with Western blots. (A) PrPSc in hamsters' brains of agent 263 K, 139A and ME7 detected with mAb 3 F4. (B) PrPSc in mice brains of agent 139A and ME7 detected with mAb 1E4. (C) Deglycosylated PrPSc in hamsters' brains of agent 263 K, 139A and ME7 detected with mAb 3 F4. (D) Immunoreactivities of PrPSc in hamsters' brains of agent 263 K, 139A and ME7 with a panel of PrP specific mAbs, including 1E4, 6D11, 3 F4, 6H4 and 8H4. Molecular weight standards are shown on the left and different mAbs are shown on the right. "+": with PK or PNGase F; "-": without PK The products of PK-digested PrPSc from the hamsters' brains infected by mouse-adapted agents 139A and ME7 were further exposed to glycosidase. One single PrP-specific bands were observed, which were at the exactly same position in SDS-PAGE as that in the preparation of 263 K PrPSc (Figure 4C). It implies that the newly-formed PrPSc in the hamsters' brains by infections of mouse-adapted scrapie agents have the same glycosylated profiles as the hamster-adapted scrapie strain 263 K.

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Virology Journal

ISSN: 1743-422X