Figure 5From: Systemic virus distribution and host responses in brain and intestine of chickens infected with low pathogenic or high pathogenic avian influenza virusSecondary chicken embryonic brain cell cultures stained with the anti-AIV nucleoprotein specific Moab HB65. Cells were cultured with LPAIV (a-d) or HPAIV (e-h) for 2 h, washed and incubated with fresh medium for 0 h. (a,e) or 24 h (b,f). From the 24 h culture 10% supernatant was transferred to fresh cultures and cultures were incubated for another 24 h without (c,g) or with (d,h) 0.005% trypsin. Magnification: 100×.Back to article page