EB reduces gene expression in CPXV and MPXV. Triplicate wells of BSC-1 cells were infected with recombinant CPXV (A) or recombinant MPXV (B) expressing GFP under the control of an artificial early/late promoter at a m.o.i. of 1 in the presence of EB for 1 hr at 4°C, rinsed 3 times, and treated with peptide-containing media. Twenty-four hours post-infection, GFP intensity was measured at 520 nm. Data are representative of three independent assays.