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Figure 5 | Virology Journal

Figure 5

From: Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein

Figure 5

7C7 does not detect coxsackieviruses. RD cells were infected with either EV71-B5 strain, CAV16, CAV10, CAV6, or CAV4. Cells were incubated 48 h until CPE was observed. Supernatants were clarified and concentrated before blotting. As a control for CAV infection, blots were labeled with enterovirus A specific MAb 4B12 which detected its targets 3D (~52 kD, arrowhead) and 3CD (~73 kD, arrowhead) in all four tested coxsackievirus strains CAV16, 10, 6 and 4 as well as EV71. However, neither VP0 nor VP2 protein was detected by MAb 7C7 in these samples (arrows).

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