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Figure 4 | Virology Journal

Figure 4

From: Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein

Figure 4

Antigenicity of VP2 is abolished in S/T(144) mutant EV71 strains. Polyclonal sera from mice injected with either wild-type EV71-B5 or EV71-C4-Fuyang RG virus were tested for the presence of anti-VP2 antibodies. Western blots were performed on samples of EV71-C4-Yamagata virus, EV71-C4-Fuyang virus, recombinant VP2-C4-Fuyang protein carrying a His tag, and recombinant VP2-B5-GST fusion protein protein. (A) Serum from EV71-B5 injected mice was used as a control. This serum could recognize VP1 in both C4 strains (arrowhead). It was also able to detect VP0 and VP2 in the EV71-C4-Yamagata stain carrying the consensus epitope EDSHP (arrows). There was no recognition of VP0 and VP2 in EV71-C4-Fuyang or of full length VP2-C4-Fuyang protein containing the S/T(144) mutation. In contrast, recombinant VP2-GST fusion protein from B5 strain was detected. (B) Serum from mice injected with EV71-C4-Fuyang was tested for the presence of anti-VP2 antibodies. Again, VP1 protein could be detected in both C4 strains (arrowheads). Additionally, there might be recognition of VP0 (arrows) but the serum contained no Abs against VP2 of either C4 virus strain, recombinant VP2-C4-Fuyang protein, or VP2-B5 protein (boxes). (C) Expression control for VP2-C4-Fuyang protein using anti-His antibody.

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