Figure 8

Fine mapping of antibody epitopes on the HPV16 capsid. (A) Same view as in Figure 7B, with the H16.H5 epitope (residues 173-185) shown in red. Side chains in the C-terminal arms of neighboring capsomeres that partially occluding the H16.H5 epitope are shown as colored spheres (not red). The conserved disulfide bond between Cys175 and Cys428 is highlighted with green spheres. The HPV16-specific Val178 is shown in black. (B) Close-up of the H16.H5 epitope from the subunit in the foreground of (A) with the same coloring scheme as in (A). (C) Same view as in (A), with the H16.V5, H16.E70, H263.A2 and H16.J4 epitopes highlighted: residues 261-265 (H16.J4 epitope only) are in dark green, residues 266-280 (in all four antibody epitopes) are in red, residues 281-297 (not part of the H16.J4 epitope) are in magenta. Residues 339-365 are in pink (not part of the H16.J4 epitope). Glu269 is shown in black. (D) Same view as in (A), with the H16.I23 epitope (residues 111-130) in red.