Figure 9

(a) Post-treatment assay. HeLa cells infected with MPXV-Zaire at an MOI = 5 in the presence or absence of 2000 U/ml of IFN-β were treated with 2000 U/ml of IFN-β at the indicated times after infection. The cells were harvested 24 h p.i. and lysates were titered by plaque assay. (b) Infection of fibroblast with MPXV. Normal human dermal fibroblasts were pre-treated for 24 h with 2000 U/ml of IFN-β. After pre-treatment, the cells were infected with MPXV-Zaire (MOI = 5) in the presence or absence of 2000 U/ml of IFN-β. The cells were harvested 24 h p.i., and the amount of virus present in the lysates was titered by plaque assay.