Figure 4From: RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cellsAnalysis effect of RNAi on ORF095 by RT-PCR. (A) Cells were harvested, and transcripts were analyzed by RT-PCR amplification. The 28S, 18S, and 5.8S rRNAs were visualized under UV light for equal loading control. (B) Specific siRNA inhibits the accumulation of ORF095 message. BHK-21 cells were transfected with pEGFP-N1 and then transfected with variant shRNA-expressing vectors. Forty hours post-transfection, total RNA was extracted and subjected to fluorescence quantitative PCR analysis. pC transfected cells and mock-transfected cells were used as controls. The mRNA of beta actin served as an internal reference.Back to article page