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Figure 3 | Virology Journal

Figure 3

From: RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells

Figure 3

Transient expression of siRNAs conferred the sequence-specific inhibition of expression of GTPV ORF95 in BKH-21 cells. (A) Fluorescence detection of cotransfection of p095/EGFP with their corresponding siRNA expression plasmids 24 h posttransfection. (B) Flow cytometric analysis of cotransfection of p095/EGFP with their corresponding siRNA expression plasmids 48 h posttransfection. EGFP expression level in cells cotransfected with (a) pEGFP-N1 vector; (b) p095/EGFP; (c) p095/EGFP and p61; (d) p095/EGFP and p70; (e) p095/EGFP and p165; (f) p095/EGFP and p296; (g) p095/EGFP and pControl; (h) BHK-21 cell control. LR-Value means the rate of EGFP positive cells.

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Virology Journal

ISSN: 1743-422X