Figure 1

Schematic representations of target construct, siRNA-expressing plasmids, and predicted siRNAs. (A)Position of target siRNAs: GTPV-ORF095-61, GTPV-ORF095-70, GTPV-ORF095-165 and GTPV-ORF095-296. (B) siRNA expressing plasmids and predicted siRNAs. An inverted repeat is inserted at the 3'-end of the human U6 promoter. The forward sequence of the repeat is 21 or 63 nt long, corresponding to the region of interest of the ORF095 gene. The forward and reverse motifs are separated by a 7-nt spacer, 5'-TCAAGAG-3'. The transcriptional termination signal of five Ts is added at the 3'-end of the inverted repeat. The synthesized RNA is predicted to fold back to form a hairpin dsRNA, which would be finally processed into the putative siRNAs.