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Table 4 Constructs, their infectivity and symptoms induced on agroinoculated plants

From: Biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in India

Test plant(s)

Construct combination used for agroinoculation

Plants showed symptom/Plants inoculated

Symptom

First symptom appearance (dpi)

N. benthamiana

RLA

7/16

LC, Ens

20

 

RLA + RLβ

12/16

LC, Ens, IVCL, Tw, Bls

8

 

RLA+VB

5/16

LC, Ens

20

 

RLA+NB

5/16

LC, Ens

20

 

VA+RLβ

16/16

LC, IVCL, CR, LD, St, BLs, SL

5

 

NA+RLβ

16/16

LC, IVCL, CR, St, BLs, SL

7

Tomato

RLA

0/16

-

-

 

RLA + RLβ

0/12

-

-

 

RLA+VB

0/12

-

-

 

RLA+NB

0/12

-

-

 

VA+RLβ

12/12

SLC, CR, LD, St, BLs, SL, LPk

8

 

NA+RLβ

7/12

LC, CR, YL, Tw

18

N. benthamiana

CRA

16/16

LC, Ens, Tw

7

 

CRA+CRβ

16/16

LC, Ens, IVCL, Bls, St, SL

6

 

CRA+VB

16/16

LC, Ens, Tw

7

 

CRA+NB

16/16

LC, Ens, Tw

7

 

CRA+ RLβ

16/16

LC, Ens, IVCL, Bls, St, SL

6

 

VA+CRβ

16/16

LC, IVCL, BLs, Tw

6

 

NA+CRβ

16/16

LC, MY, VC, Bls,

6

 

CRA+VA+CRβ

16/16

LC, IVCL, CR, Ens, St, BLs, Tw

5

 

CRA+NA+CRβ

16/16

LC, Bls, IVCL, Ens, Tw

5

 

CRA+VA+VB

16/16

LC, IVCL, CR, Ens, Chls, BLs, SL, Tw

5

 

CRA+NA+NB

16/16

LC, YL, VB, St, Mt, Bls, IVCL, Ens

5

 

CRA+VA+VB+β

16/16

LC, IVCL, Ens, LD, St, Bls, SL, Tw

5

 

CRA+NA+NB+β

16/16

SL, YL, St, LC, Mt, Tw

5

 

VA+VB

10/10

LC, IVCL, CR, LD, Chls, Bls, SL

6

 

NA+NB

10/10

SL, YL, LC, Mt

5

Tomato

CRA

10/12

LC, LR

21

 

CRA+CRβ

12/12

LC, LR, VC, SL

18

 

CRA+VB

10/12

LC, LR

21

 

CRA+NB

9/12

LC, LR

21

 

CRA+ RLβ

12/12

LC, LR, VC, SL

19

 

NA+CRβ

0/12

-

-

 

VA+CRβ

10/12

LC, MY

9

 

CRA+VA+CRβ

12/12

LC, LR, VC, SL, MY

9

 

CRA+NA+CRβ

12/12

LC, LR, VC, SL

16

 

CRA+VA+VB

12/12

LC, LR, CR, YL

7

 

CRA+NA+NB

12/12

LC, LR, VC, SL, YL

8

 

CRA+VA+VB+β

12/12

LC, LR, CR, YL, St

7

 

CRA+NA+NB+β

12/12

LC, LR, VC, SL, YL

8

 

VA+VB

10/10

LC, CR, LR, YL

7

 

NA+NB

10/10

LC, CR, YL

8

  1. LC leaf curling; BV big veins; MY mild yellowing; YL yellow leaf lamina; St stunting; LD leaf distortion; VB vein banding; MO severe mosaic; LR leaf rolling; Bls blistering; SL small leaves; CR leaf crinkling; IVCL interveinal chlorosis; Chls chlorotic spots; MiMO mild mosaic; Mt mottling; SYL severe yellow leaf lamina; Tw twisting of petiole VC vein clearing, LPk Leaf puckering, Ens enation